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Substrate stiffness upregulates ALKBH1. ( A ) Histograms of mRNA expression of ALKBH1, METTL4 and <t>N6AMT1</t> in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate. ( B ) Western blot of ALKBH1, METTL4 and N6AMT1 in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate, using GAPDH as a control. ( C-D ) IF staining indicated ALKBH1 levels of HCT116 cells ( C ) and RKO cells ( D ) stimulated by soft and stiff substrate. ( E-F ) Western blot ( E ) and qRT-PCR (F) of ALKBH1, METTL4 and N6AMT1 in orthotopic xenograft model treated by PBS and BAPN, using GAPDH as a control. ( G-H ) Western blot ( G ) and qRT-PCR (H) of ALKBH1, METTL4 and N6AMT1 in DSS/AOM-induced CRC model treated by PBS and BAPN, using GAPDH as a control. ( I-J ) Representative IHC (left panel) and relative quantitative statistics (right panel) of ALKBH1 in orthotopic xenograft model ( I ) and DSS/AOM-induced CRC model ( J ) treated by PBS and BAPN. ( K ) Representative IHC of ALKBH1 in low collagen I group and high collagen I group of human CRC tissues. ( L ) Spearman’s correlation analysis of ALKBH1 IHC scores and collagen I IHC scores in human CRC tissues. ( M ) Representative IHC of ALKBH1 in low stiffness group and high stiffness group of DSS/AOM induced CRC mice. ( N ) Spearman’s correlation analysis of ALKBH1 IHC scores and elastic modulus in AOM/DSS-induced CRC tissues Data are represented as mean ± SEM. * P < 0.05; ns, not significant
Anti N6amt1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti n6amt1 antibody - by Bioz Stars, 2026-02
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Substrate stiffness upregulates ALKBH1. ( A ) Histograms of mRNA expression of ALKBH1, METTL4 and <t>N6AMT1</t> in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate. ( B ) Western blot of ALKBH1, METTL4 and N6AMT1 in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate, using GAPDH as a control. ( C-D ) IF staining indicated ALKBH1 levels of HCT116 cells ( C ) and RKO cells ( D ) stimulated by soft and stiff substrate. ( E-F ) Western blot ( E ) and qRT-PCR (F) of ALKBH1, METTL4 and N6AMT1 in orthotopic xenograft model treated by PBS and BAPN, using GAPDH as a control. ( G-H ) Western blot ( G ) and qRT-PCR (H) of ALKBH1, METTL4 and N6AMT1 in DSS/AOM-induced CRC model treated by PBS and BAPN, using GAPDH as a control. ( I-J ) Representative IHC (left panel) and relative quantitative statistics (right panel) of ALKBH1 in orthotopic xenograft model ( I ) and DSS/AOM-induced CRC model ( J ) treated by PBS and BAPN. ( K ) Representative IHC of ALKBH1 in low collagen I group and high collagen I group of human CRC tissues. ( L ) Spearman’s correlation analysis of ALKBH1 IHC scores and collagen I IHC scores in human CRC tissues. ( M ) Representative IHC of ALKBH1 in low stiffness group and high stiffness group of DSS/AOM induced CRC mice. ( N ) Spearman’s correlation analysis of ALKBH1 IHC scores and elastic modulus in AOM/DSS-induced CRC tissues Data are represented as mean ± SEM. * P < 0.05; ns, not significant
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Substrate stiffness upregulates ALKBH1. ( A ) Histograms of mRNA expression of ALKBH1, METTL4 and <t>N6AMT1</t> in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate. ( B ) Western blot of ALKBH1, METTL4 and N6AMT1 in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate, using GAPDH as a control. ( C-D ) IF staining indicated ALKBH1 levels of HCT116 cells ( C ) and RKO cells ( D ) stimulated by soft and stiff substrate. ( E-F ) Western blot ( E ) and qRT-PCR (F) of ALKBH1, METTL4 and N6AMT1 in orthotopic xenograft model treated by PBS and BAPN, using GAPDH as a control. ( G-H ) Western blot ( G ) and qRT-PCR (H) of ALKBH1, METTL4 and N6AMT1 in DSS/AOM-induced CRC model treated by PBS and BAPN, using GAPDH as a control. ( I-J ) Representative IHC (left panel) and relative quantitative statistics (right panel) of ALKBH1 in orthotopic xenograft model ( I ) and DSS/AOM-induced CRC model ( J ) treated by PBS and BAPN. ( K ) Representative IHC of ALKBH1 in low collagen I group and high collagen I group of human CRC tissues. ( L ) Spearman’s correlation analysis of ALKBH1 IHC scores and collagen I IHC scores in human CRC tissues. ( M ) Representative IHC of ALKBH1 in low stiffness group and high stiffness group of DSS/AOM induced CRC mice. ( N ) Spearman’s correlation analysis of ALKBH1 IHC scores and elastic modulus in AOM/DSS-induced CRC tissues Data are represented as mean ± SEM. * P < 0.05; ns, not significant
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Substrate stiffness upregulates ALKBH1. ( A ) Histograms of mRNA expression of ALKBH1, METTL4 and <t>N6AMT1</t> in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate. ( B ) Western blot of ALKBH1, METTL4 and N6AMT1 in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate, using GAPDH as a control. ( C-D ) IF staining indicated ALKBH1 levels of HCT116 cells ( C ) and RKO cells ( D ) stimulated by soft and stiff substrate. ( E-F ) Western blot ( E ) and qRT-PCR (F) of ALKBH1, METTL4 and N6AMT1 in orthotopic xenograft model treated by PBS and BAPN, using GAPDH as a control. ( G-H ) Western blot ( G ) and qRT-PCR (H) of ALKBH1, METTL4 and N6AMT1 in DSS/AOM-induced CRC model treated by PBS and BAPN, using GAPDH as a control. ( I-J ) Representative IHC (left panel) and relative quantitative statistics (right panel) of ALKBH1 in orthotopic xenograft model ( I ) and DSS/AOM-induced CRC model ( J ) treated by PBS and BAPN. ( K ) Representative IHC of ALKBH1 in low collagen I group and high collagen I group of human CRC tissues. ( L ) Spearman’s correlation analysis of ALKBH1 IHC scores and collagen I IHC scores in human CRC tissues. ( M ) Representative IHC of ALKBH1 in low stiffness group and high stiffness group of DSS/AOM induced CRC mice. ( N ) Spearman’s correlation analysis of ALKBH1 IHC scores and elastic modulus in AOM/DSS-induced CRC tissues Data are represented as mean ± SEM. * P < 0.05; ns, not significant
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Substrate stiffness upregulates ALKBH1. ( A ) Histograms of mRNA expression of ALKBH1, METTL4 and N6AMT1 in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate. ( B ) Western blot of ALKBH1, METTL4 and N6AMT1 in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate, using GAPDH as a control. ( C-D ) IF staining indicated ALKBH1 levels of HCT116 cells ( C ) and RKO cells ( D ) stimulated by soft and stiff substrate. ( E-F ) Western blot ( E ) and qRT-PCR (F) of ALKBH1, METTL4 and N6AMT1 in orthotopic xenograft model treated by PBS and BAPN, using GAPDH as a control. ( G-H ) Western blot ( G ) and qRT-PCR (H) of ALKBH1, METTL4 and N6AMT1 in DSS/AOM-induced CRC model treated by PBS and BAPN, using GAPDH as a control. ( I-J ) Representative IHC (left panel) and relative quantitative statistics (right panel) of ALKBH1 in orthotopic xenograft model ( I ) and DSS/AOM-induced CRC model ( J ) treated by PBS and BAPN. ( K ) Representative IHC of ALKBH1 in low collagen I group and high collagen I group of human CRC tissues. ( L ) Spearman’s correlation analysis of ALKBH1 IHC scores and collagen I IHC scores in human CRC tissues. ( M ) Representative IHC of ALKBH1 in low stiffness group and high stiffness group of DSS/AOM induced CRC mice. ( N ) Spearman’s correlation analysis of ALKBH1 IHC scores and elastic modulus in AOM/DSS-induced CRC tissues Data are represented as mean ± SEM. * P < 0.05; ns, not significant

Journal: Experimental Hematology & Oncology

Article Title: Extracellular matrix stiffness reduces DNA 6 ma level to facilitate colorectal cancer progression via disrupting P53 binding to CDKN1A promoter

doi: 10.1186/s40164-025-00704-w

Figure Lengend Snippet: Substrate stiffness upregulates ALKBH1. ( A ) Histograms of mRNA expression of ALKBH1, METTL4 and N6AMT1 in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate. ( B ) Western blot of ALKBH1, METTL4 and N6AMT1 in HCT116 (left panel) and RKO cells (right panel) stimulated by soft and stiff substrate, using GAPDH as a control. ( C-D ) IF staining indicated ALKBH1 levels of HCT116 cells ( C ) and RKO cells ( D ) stimulated by soft and stiff substrate. ( E-F ) Western blot ( E ) and qRT-PCR (F) of ALKBH1, METTL4 and N6AMT1 in orthotopic xenograft model treated by PBS and BAPN, using GAPDH as a control. ( G-H ) Western blot ( G ) and qRT-PCR (H) of ALKBH1, METTL4 and N6AMT1 in DSS/AOM-induced CRC model treated by PBS and BAPN, using GAPDH as a control. ( I-J ) Representative IHC (left panel) and relative quantitative statistics (right panel) of ALKBH1 in orthotopic xenograft model ( I ) and DSS/AOM-induced CRC model ( J ) treated by PBS and BAPN. ( K ) Representative IHC of ALKBH1 in low collagen I group and high collagen I group of human CRC tissues. ( L ) Spearman’s correlation analysis of ALKBH1 IHC scores and collagen I IHC scores in human CRC tissues. ( M ) Representative IHC of ALKBH1 in low stiffness group and high stiffness group of DSS/AOM induced CRC mice. ( N ) Spearman’s correlation analysis of ALKBH1 IHC scores and elastic modulus in AOM/DSS-induced CRC tissues Data are represented as mean ± SEM. * P < 0.05; ns, not significant

Article Snippet: Anti-PCNA antibody (10205-2-AP), Anti-ALKBH1 antibody (27973-1-AP), Anti-N6AMT1 antibody (16211-1-AP), and Anti-GAPDH antibody (10494-1-AP) were purchased from Proteintech.

Techniques: Expressing, Western Blot, Control, Staining, Quantitative RT-PCR